Hiroshi Ishikita 研究室

主宰者：Hiroshi Ishikita

東京大学

AI 要約（直近 5 年の研究成果）

本研究室は、光合成や視覚など、生命現象に関わるタンパク質内での光エネルギー変換と電子移動の仕組みを、分子レベルで解き明かすことを目指しています。特に、光を吸収する色素分子がタンパク質環境内でどのような構造をとり、その周囲の化学環境がどう色や反応性に影響するのかという問題に取り組んでいます。例えば、光合成に関わる様々なタンパク質が、同じ色素分子を使用していても異なる波長の光を吸収する理由、あるいは光エネルギーから電子移動へと変換される過程における正確なメカニズムなどが主要な研究対象です。研究の手法としては、量子化学計算とタンパク質の分子シミュレーションを組み合わせた計算アプローチを活用しています。これらの手法により、結晶構造解析では直接観察できない水素結合ネットワークやプロトン移動の経路、電子の移動ルートといった動的な分子過程を可視化できます。また、NMR分光やX線構造解析などの実験データと計算結果を相互検証することで、タンパク質内での化学反応の正確な理解へと進んでいます。これらの研究成果は、光合成の効率化や新しい生体センサー・医療診断技術の開発へも応用できる基礎知見を提供しています。さらに、遺伝子変異に由来するタンパク質機能障害の分子的原因の解明にも取り組み、医療的な課題の解決にも貢献する研究室です。

※ AI（Claude）が、公開されている論文要旨から研究の問い・手法・主要な発見を事実情報として抽出・再構成して自動生成しています。誤りを含む可能性があるため、正確性は研究室公式情報でご確認ください。

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研究成果（77 件）

[2026] Molecular basis for alternative charge separation pathways in type-II photosynthetic reaction centers
DOI: https://doi.org/10.1093/pnasnexus/pgag111
[2026] Mechanisms of Red-Shifted Absorption in Biliverdin-Binding Proteins
DOI: https://doi.org/10.1021/acs.biochem.6c00202
[2026] Rhodopsin 7 is Indispensable for Regulating the Firing Rates of Olfactory Sensory Neurons in Response to Extracellular Field Potential Changes in <scp> Drosophila melanogaster </scp>
DOI: https://doi.org/10.1111/ejn.70509
[2025] Identification and design principles of far-red–absorbing chlorophyll in the light-harvesting complex
DOI: https://doi.org/10.1016/j.jbc.2025.108518
[2025] Structural insights into lipid chain-length selectivity and allosteric regulation of FFA2
DOI: https://doi.org/10.1038/s41467-025-57983-4
[2025] Roles of basic amino acid residues in substrate binding and transport of the light-driven anion pump Synechocystis halorhodopsin (SyHR)
DOI: https://doi.org/10.1016/j.jbc.2025.108334
[2025] Mechanistic insights into glycosylation-driven structural rearrangements in human aquaporin 1
DOI: https://doi.org/10.1039/d5cp01753j
[2025] Stretching Vibrational Frequencies of Hydrogen Bond Networks and Proton Transfer in Protein Environments
DOI: https://doi.org/10.2142/biophys.65.305
[2025] Revisiting the Proposed Protonated Water Cluster at the Exit Site of the Proton Transfer Pathway in Bacteriorhodopsin
DOI: https://doi.org/10.1021/acs.jpcb.5c03321
[2025] Redox potentials of carotenoids associated with type-II photosynthetic reaction centers
DOI: https://doi.org/10.1093/pcp/pcaf167

続きを表示（残り 67 件）

[2025] A DNA2 mutation in the ATP-binding motif identified in a diagnostically unresolved individual
DOI: https://doi.org/10.3389/fmolb.2025.1706392
[2025] Current perspectives on proton and electron transfer pathways in photosystem II water oxidation
DOI: https://doi.org/10.1093/pcp/pcaf107
[2025] Energetics of tryptophan residues in electron transfer and photoprotection of type-II photosynthetic reaction centers
DOI: https://doi.org/10.1093/pnasnexus/pgaf278
[2025] Electron Transfer Mechanism from the Oxygen-Evolving Complex to the Electron-Acceptor Tyrosine during the S2 to S3 Transition in Photosystem II
DOI: https://doi.org/10.1021/acsomega.5c00945
[2025] Distinct Protochromic Mechanisms Driving Green/Red Absorption in Phycocyanobilin-Binding Proteins
DOI: https://doi.org/10.1021/acs.biochem.4c00870
[2025] Origin of the unique topology of the triangular water cluster in Rubrobacter xylanophilus rhodopsin
DOI: https://doi.org/10.2142/biophysico.bppb-v22.0018
[2025] Determinants of hydrogen bond distances in proteins
DOI: https://doi.org/10.1039/d5cp00511f
[2024] D1-Tyr246 and D2-Tyr244 in photosystem II: Insights into bicarbonate binding and electron transfer from QA•− to QB
DOI: https://doi.org/10.1016/j.bbabio.2024.149507
[2024] Exploring the Deprotonation Process during Incorporation of a Ligand Water Molecule at the Dangling Mn Site in Photosystem II
DOI: https://doi.org/10.1021/acs.jpcb.4c01997
[2024] Modulation of Electron Transfer Branches by Atrazine and Triazine Herbicides in Photosynthetic Reaction Centers
DOI: https://doi.org/10.1021/acs.biochem.4c00010
[2024] Energetics of the H-Bond Network in Exiguobacterium sibiricum Rhodopsin
DOI: https://doi.org/10.1021/acs.biochem.4c00182
[2024] Molecular origins of absorption wavelength variation among phycocyanobilin-binding proteins
DOI: https://doi.org/10.1016/j.bpj.2024.08.001
[2024] ZBTB7A forms a heterodimer with ZBTB2 and inhibits ZBTB2 homodimerization required for full activation of HIF-1
DOI: https://doi.org/10.1016/j.bbrc.2024.150604
[2024] Organic solvent-induced structural changes in a protein confined in a giant coordination cage
DOI: https://doi.org/10.1093/chemle/upae101
[2024] How the Electron-Transfer Cascade is Maintained in Chlorophyll- d Containing Photosystem I
DOI: https://doi.org/10.1021/acs.biochem.4c00521
[2024] Substrate specificity controlled by the exit site of human P4-ATPases, revealed by de novo point mutations in neurological disorders
DOI: https://doi.org/10.1073/pnas.2415755121
[2024] Superexchange Electron Transfer and Protein Matrix in the Charge-Separation Process of Photosynthetic Reaction Centers
DOI: https://doi.org/10.1021/acs.jpclett.4c02232
[2024] Absence of a link between stabilized charge-separated state and structural changes proposed from crystal structures of a photosynthetic reaction center
DOI: https://doi.org/10.1038/s42004-024-01281-5
[2024] Direct evidence for a deprotonated lysine serving as a H-bond “acceptor” in a photoreceptor protein
DOI: https://doi.org/10.1073/pnas.2404472121
[2023] Interplay of two low-barrier hydrogen bonds in long-distance proton-coupled electron transfer for water oxidation
DOI: https://doi.org/10.1093/pnasnexus/pgad423
[2023] Structural and energetic insights into Mn-to-Fe substitution in the oxygen-evolving complex
DOI: https://doi.org/10.1016/j.isci.2023.107352
[2023] Difference in the Charge-Separation Energetics between Distinct Conformers in the PixD Photoreceptor
DOI: https://doi.org/10.1021/acs.jpcb.3c06483
[2023] Characterization of tryptophan oxidation affecting D1 degradation by FtsH in the photosystem II quality control of chloroplasts
DOI: https://doi.org/10.7554/elife.88822.3
[2023] Stretching vibrational frequencies and pKa differences in H-bond networks of protein environments
DOI: https://doi.org/10.1016/j.bpj.2023.10.012
[2023] Absence of electron-transfer-associated changes in the time-dependent X-ray free-electron laser structures of the photosynthetic reaction center
DOI: https://doi.org/10.7554/elife.88955.4
[2023] pH-Dependent Binding and Releasing Mechanism of Acetate in the Inner Water Cavity of Heliorhodopsin
DOI: https://doi.org/10.1021/acs.biochem.3c00193
[2023] Absence of electron-transfer-associated changes in the time-dependent X-ray free-electron laser structures of the photosynthetic reaction center
DOI: https://doi.org/10.7554/elife.88955
[2023] Insights into the protonation state and spin structure for the g = 2 multiline electron paramagnetic resonance signal of the oxygen-evolving complex
DOI: https://doi.org/10.1093/pnasnexus/pgad244
[2023] Characterization of tryptophan oxidation affecting D1 degradation by FtsH in the photosystem II quality control of chloroplasts
DOI: https://doi.org/10.7554/elife.88822
[2023] Redox Potentials of Iron–Sulfur Clusters in Type I Photosynthetic Reaction Centers
DOI: https://doi.org/10.1021/acs.jpcb.3c01071
[2023] Mechanism of Asparagine-Mediated Proton Transfer in Photosynthetic Reaction Centers
DOI: https://doi.org/10.1021/acs.biochem.3c00013
[2023] Identification of the protonation and oxidation states of the oxygen-evolving complex in the low-dose X-ray crystal structure of photosystem II
DOI: https://doi.org/10.3389/fpls.2023.1029674
[2023] Energetic Diversity in the Electron-Transfer Pathways of Type I Photosynthetic Reaction Centers
DOI: https://doi.org/10.1021/acs.biochem.2c00689
[2023] Mechanism of Absorption Wavelength Shift Depending on the Protonation State of the Acrylate Group in Chlorophyll c
DOI: https://doi.org/10.1021/acs.jpcb.2c07232
[2023] Quantum mechanical analysis of excitation energy transfer couplings in photosystem II
DOI: https://doi.org/10.1016/j.bpj.2023.01.002
[2023] Substitution of Ca2+ and changes in the H-bond network near the oxygen-evolving complex of photosystem II
DOI: https://doi.org/10.1039/d2cp05036f
[2022] A self-assembled coordination cage enhances the reactivity of confined amides via mechanical bond-twisting
DOI: https://doi.org/10.1039/d2cp03126d
[2022] Proton transfer and conformational changes along the hydrogen bond network in heliorhodopsin
DOI: https://doi.org/10.1038/s42003-022-04311-x
[2022] A Basified Phenothiazine at Submicromolar Level Boosts Microbial Electrocatalysis by Two Orders of Magnitude
DOI: https://doi.org/10.2139/ssrn.4044112
[2022] ZBTB2 links p53 deficiency to HIF‐1‐mediated hypoxia signaling to promote cancer aggressiveness
DOI: https://doi.org/10.15252/embr.202154042
[2022] Energetics of the Electron Transfer Pathways in the Homodimeric Photosynthetic Reaction Center
DOI: https://doi.org/10.1021/acs.biochem.2c00524
[2022] Protonation structure of the closed-cubane conformation of the O2-evolving complex in photosystem II
DOI: https://doi.org/10.1093/pnasnexus/pgac221
[2022] Proton-mediated photoprotection mechanism in photosystem II
DOI: https://doi.org/10.3389/fpls.2022.934736
[2022] Conformational Changes and H-Bond Rearrangements during Quinone Release in Photosystem II
DOI: https://doi.org/10.1021/acs.biochem.2c00324
[2022] Electron-Transfer Route in the Early Oxidation States of the Mn4CaO5 Cluster in Photosystem II
DOI: https://doi.org/10.1021/acs.jpcb.2c08246
[2022] D139N mutation of PsbP enhances the oxygen-evolving activity of photosystem II through stabilized binding of a chloride ion
DOI: https://doi.org/10.1093/pnasnexus/pgac136
[2022] Correlation between C═O Stretching Vibrational Frequency and pKa Shift of Carboxylic Acids
DOI: https://doi.org/10.1021/acs.jpcb.2c02193
[2022] Release of a Proton and Formation of a Low-Barrier Hydrogen Bond between Tyrosine D and D2-His189 in Photosystem II
DOI: https://doi.org/10.1021/acsphyschemau.2c00019
[2022] Release of Electrons and Protons from Substrate Water Molecules at the Oxygen-Evolving Complex in Photosystem II
DOI: https://doi.org/10.7566/jpsj.91.091012
[2022] Mechanism of Mixed-Valence Fe2.5+···Fe2.5+ Formation in Fe4S4 Clusters in the Ferredoxin Binding Motif
DOI: https://doi.org/10.1021/acs.jpcb.2c01320
[2022] Absorption wavelength along chromophore low-barrier hydrogen bonds
DOI: https://doi.org/10.1016/j.isci.2022.104247
[2022] Structure-guided design enables development of a hyperpolarized molecular probe for the detection of aminopeptidase N activity in vivo
DOI: https://doi.org/10.1126/sciadv.abj2667
[2022] Mechanism of Absorption Wavelength Shift of Bacteriorhodopsin During Photocycle
DOI: https://doi.org/10.1021/acs.jpcb.2c04359
[2022] Electron Transfer Route between Quinones in Type-II Reaction Centers
DOI: https://doi.org/10.1021/acs.jpcb.2c05713
[2021] Requirement of Chloride for the Downhill Electron Transfer Pathway from the Water-Splitting Center in Natural Photosynthesis
DOI: https://doi.org/10.1021/acs.jpcb.1c09176
[2021] Exploring the Retinal Binding Cavity of Archaerhodopsin-3 by Replacing the Retinal Chromophore With a Dimethyl Phenylated Derivative
DOI: https://doi.org/10.3389/fmolb.2021.794948
[2021] Long-Range Electron Tunneling from the Primary to Secondary Quinones in Photosystem II Enhanced by Hydrogen Bonds with a Nonheme Fe Complex
DOI: https://doi.org/10.1021/acs.jpcb.1c09538
[2021] Structural basis for high selectivity of a rice silicon channel Lsi1
DOI: https://doi.org/10.1038/s41467-021-26535-x
[2021] A sublethal ATP11A mutation associated with neurological deterioration causes aberrant phosphatidylcholine flipping in plasma membranes
DOI: https://doi.org/10.1172/jci148005
[2021] Electron Acceptor–Donor Iron Sites in the Iron–Sulfur Cluster of Photosynthetic Electron-Transfer Pathways
DOI: https://doi.org/10.1021/acs.jpclett.1c01896
[2021] Identification of intermediate conformations in the photocycle of the light-driven sodium-pumping rhodopsin KR2
DOI: https://doi.org/10.1016/j.jbc.2021.100459
[2021] The origin of unidirectional charge separation in photosynthetic reaction centers: nonadiabatic quantum dynamics of exciton and charge in pigment–protein complexes
DOI: https://doi.org/10.1039/d1sc01497h
[2021] Nature of Asymmetric Electron Transfer in the Symmetric Pathways of Photosystem I
DOI: https://doi.org/10.1021/acs.jpcb.0c10885
[2021] Two Distinct Oxygen-Radical Conformations in the X-ray Free Electron Laser Structures of Photosystem II
DOI: https://doi.org/10.1021/acs.jpclett.1c00814
[2021] Role of redox-inactive metals in controlling the redox potential of heterometallic manganese–oxido clusters
DOI: https://doi.org/10.1007/s11120-021-00846-y
[2021] Mechanism of the formation of proton transfer pathways in photosynthetic reaction centers
DOI: https://doi.org/10.1073/pnas.2103203118
[2021] Absorption Wavelength Along Chromophore Low-Barrier Hydrogen Bonds
DOI: https://doi.org/10.2139/ssrn.3908775

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AI 要約（直近 5 年の研究成果）

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研究成果（77 件）

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